Long-read pricing, gCore

Feb. 2025. Prices are subject to change. 

Prices for internal (int.) and external (ext.) customers (definitions).

All prices include labor and supplies. Bioinformatics is charged separately.

PacBio Revio long-read DNA Sequencing

• Optional HMW DNA extraction: int. $135 (ext. $203)

• Library QC & Prep: int. $600 (ext. $900)

• Sequencing per SMRT cell (90+ Gbp): int. $1600 (ext. $2400)

• 3-sample multiplexing package: int. $1268 (ext. $1902) per sample includes DNA extraction, library prep, sequencing, and demultiplexing. Output 30Gbps per sample = 10X coverage for 3Gb genome

PacBio Revio long-read RNA Sequencing

Bulk RNA IsoSeq (8-sample batch):

• •  int. $488 (ext. $732) per sample (5M+ reads) or

  •  int. $688 (ext. $1032) per sample (10M+ reads). 

Includes library prep and sequencing

Additional services for de novo genome sequencing

• Nanopore ultra-long sequencing: int. $2135 (ext. $3203) per sample includes DNA extraction, ultra-long library prep (>50kb), and sequencing.

• Hi-C sequencing: int. $1600 (ext. $2400) per sample. Includes library prep and sequencing. Estimated output 600M PE150 reads. Hi-C helps the assembly of full chromosomes from contigs.

• Bionano optical chromosome mapping (min 3 samples): int. $2267 (ext. $3400) per sample. Includes DNA extraction, library prep, and mapping. Output 40Gb at 400X coverage, fragment size 300kb-1Mb+

Bioinformatics analysis

int. $107 (ext. $160) per hour. Services based on PacBio long-read sequencing data include:

• De novo genome assembly, based on long-read PacBio, Nanopore, Hi-C, and Bionano

• Genome annotation based on long-read RNA IsoSeq

• Full genome SNP detection and phasing (int. $300/ext. $450 per sample, in 4-sample batch)

• Transgene detection and structural variants (int. $300/ext. $450 per sample, in 4-sample batch)

• Methylation analysis