Olink Proteomics Service

gCore will soon offer proteomics service using the Reveal panel from Olink Proteomics, a part of Thermo Fisher Scientific

Service Overview

• Technology: Proximity Extension Assay (PEA) with next-generation sequencing (NGS)

• Panel: Reveal 1034 – Measures 1,034 human protein markers (537 inflammation-focused; 700+ linked to genetic variants)

• Sample Requirements:

  • Volume: 10 µL (4 µL used per assay; excess ensures technical reliability)

  • Compatible sample types: Plasma, serum, CSF, urine, tissue lysate, saliva, synovial fluid, NHP plasma/serum

Pricing

• Cost: $173/sample (includes library preparation + sequencing in 86-sample batches)

• Batch Flexibility: Split-run participation available via waitlist signup. Although the 86-sample batch must be processed at once, we offer researchers the option to split it. If you're interested, please sign up for our batch splitting waitlist. Once we have enough signups, we'll contact you to submit your samples.

Performance Highlights

• Sensitivity: Detects proteins down to fg/mL concentrations

• Precision: Intra-plate CV: 8.1% | Inter-site CV: 6.3%

• Reproducibility: Concordance with Olink Explore HT (R=0.95) | Between-site correlation R=0.96–0.98

How It Works

1. Prepare: Collect samples following Olink guidelines

2. Submit: Ship to gCore with completed metadata forms

3. Analyze: Receive Normalized Protein Expression (NPX) data within 2 months

Contact: gcore@health.ucsd.edu