Olink Proteomics Service

gCore will soon offer proteomics service using the Reveal panel from Olink Proteomics, a part of Thermo Fisher Scientific

Service Overview

• Technology: Proximity Extension Assay (PEA) with next-generation sequencing (NGS)

• Panel: Reveal 1034 – Measures 1,034 human protein markers (537 inflammation-focused; 700+ linked to genetic variants)

• Sample Requirements:

  • Volume: 10 µL (4 µL used per assay; excess ensures technical reliability)

  • Compatible sample types: Plasma, serum, CSF, urine, tissue lysate, saliva, synovial fluid, NHP plasma/serum

Pricing

• Cost: $173/sample (includes library preparation + sequencing in 86-sample batches)

• Batch Flexibility: Split-run participation available via waitlist signup. Although the 86-sample batch must be processed at once, we offer researchers the option to split it. If you're interested please contact Trevor Biddle at tbiddle@health.ucsd.edu to be added to the batch list. Once we have enough signups, we'll contact you to submit your samples.

Performance Highlights

• Sensitivity: Detects proteins down to fg/mL concentrations

• Precision: Intra-plate CV: 8.1% | Inter-site CV: 6.3%

• Reproducibility: Concordance with Olink Explore HT (R=0.95) | Between-site correlation R=0.96–0.98

How It Works

1. Prepare: Collect samples following Olink guidelines

2. Submit: Ship to gCore with completed metadata forms

3. Analyze: Receive Normalized Protein Expression (NPX) data within 2 months

Contact: gcore@health.ucsd.edu