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Telomere length analysis is available via long-read NanoPore and PacBio sequencing.
Telomere sequencing service using Nanopore TeloSeq
Telomere sequencing service using Nanopore TeloSeq
Nanopore TeloSeq is a new method that sequences telomeric and peritelomeric regions. The recommended starting amount of tissue is preferably 15ug of high molecular weight DNA.
TeloSeq service pricing (UCSD rates)
TeloSeq service pricing (UCSD rates)
DNA extraction service - $135 per sample.
Library prep - $400 per sample
Nanopore sequencing - $1100 per sample
TOTAL: $1635 per sample
Sholes, Samantha L., et al. "Chromosome-specific telomere lengths and the minimal functional telomere revealed by nanopore sequencing." Genome research 32.4 (2022): 616-628.
Bioinformatic measurement of telomere sequences based on PacBio Revio full genome sequencing.
Bioinformatic measurement of telomere sequences based on PacBio Revio full genome sequencing.
Human telomeres are 5-15 Kb long, and rodent telomeres are 30-100 Kb long. The telomere sequence is enriched with GGGTTA repeats, but telomere sequences are quite variable and contain enough variability to allow for using long reads to measure telomere lengths by aligning reads to the T2T sequence. PacBio Revio whole genome sequencing includes the telomeres and allows for the measurement of telomere length from the obtained whole genome sequence.
Pricing for the long-read DNA sequencing using Revio. Additional bioinformatic analysis for extraction of telomere lengths is offered.
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